Journal: European Journal of Immunology

Article Title: Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections

doi: 10.1002/eji.202048978

Figure Lengend Snippet: Type‐I Interferon quantification using FilmArray ® platform. (A) Spearman correlation [IC95%] between ISG score as defined by a 6‐interferon stimulated gene transcriptional signature using Nanostring ® platform and ISG score as defined by a 4‐interferon stimulated gene transcriptional signature using FilmArray ® technology, obtained from whole blood from (i) febrile infants (n = 86 patients from one experiment, green circle) with proven infections attending pediatric emergency departments, (ii) patients with severe infection caused by SARS‐CoV2 hospitalized in the ICU (n = 26 patients from one experiment, red square), and (iii) healthcare workers with COVID‐19 with mild symptoms (n = 7 patients from one experiment, blue triangle) (ρ = 0.991). (B) Spearman correlation between ISG score as defined by a 4‐interferon stimulated gene transcriptional signature using FilmArray ® technology and IFN‐α2 concentrations measured by SIMOA ® platform, obtained from whole blood from (i) febrile infants (n = 56 patients from one experiment, green circle) with proven infections attending pediatric emergency departments, (ii) patients with severe infection caused by SARS‐CoV2 hospitalized in the ICU (n = 16 patients from one experiment, red square), and (iii) healthcare workers with COVID‐19 with mild symptoms (n = 7 patients from one experiment, blue triangle) (ρ = 0.832). Patients are represented by different forms and nonlinear regression (second order polynomial, quadratic) is represented by a black line. IFN (Interferon), ISG (Interferon Stimulated Genes).

Article Snippet: The PAXgene TM blood samples were mixed with 800 μL of the Sample Buffer provided with the kit and directly injected into the pouch and ran on FilmArray ® 2.0 and FilmArray ® Torch instruments (BioFire Diagnostics, LLC., Salt Lake City, UT).

Techniques: Infection

Journal: European Journal of Immunology

Article Title: Type‐I Interferon assessment in 45 minutes using the FilmArray ® PCR platform in SARS‐CoV‐2 and other viral infections

doi: 10.1002/eji.202048978

Figure Lengend Snippet: Type‐I IFN assessment in COVID‐19 critically ill patients using FilmArray ® platform. ISG score as defined by a 4 interferon stimulated gene transcriptional signature was determined by IFN pouch FilmArray ® panel, obtained from whole blood from selected patients severely infected by SARS‐CoV‐2 at ±5 days after admission in the ICU (n = 16 patients from one experiment) and healthy volunteers (n = 10 donors from one experiment). COVID‐19 critically‐ill patients are represented by a black dot, and these samples are stratified according to their impairment of IFN response. The healthy volunteers (n = 10 donors from one experiment) are represented by a black dot also. The dashed line represents the arbitrary threshold of the ISG score set to separate patients with a positive (n = 4 patients from one experiment) or negative (n = 12 patients from one experiment) IFN response. The box‐and‐whisker plots represent the median and min‐max; Mann–Whitney U test ** p < 0.01; **** p < 0.0001. IFN (Interferon), ISG (Interferon Stimulated Genes).

Article Snippet: The PAXgene TM blood samples were mixed with 800 μL of the Sample Buffer provided with the kit and directly injected into the pouch and ran on FilmArray ® 2.0 and FilmArray ® Torch instruments (BioFire Diagnostics, LLC., Salt Lake City, UT).

Techniques: Infection, Whisker Assay, MANN-WHITNEY